Course unit code:
Course unit title:
Laboratory of Instrumental Methods in Analysis
Mode of completion and Number of ECTS credits:
Class req (3 credits)
Course supervisor:
doc. Ing. Katarína Hroboňová, PhD.
Learning outcomes of the course unit:
Provide advanced work using laboratory analytical techniques, basics of proper implementation of the analytical operations, good laboratory practice. Know the principles of creation of analytic signal measuring instruments and principles of optimization of various modes of measurement. Control instrumental analytical procedures for obtaining information and procedures evaluation of measurement results.
Prerequisites for registration:
Course contents:
1.Basic principles work in the analytical laboratory . Working with measures of weight and
volume. Preparation of solutions.

2.Optical methods -  
● Molecular absorption spectral analysis in visible and ultraviolet radiation
- spectrophotometric determination of salicylic acid in Acylpyrine
- spectrophotometric determination of iron in mineral water.
- spectrophotometric determination of nitrates in the UV spectrum
- spectrophotometric determination of copper
● Atomic emission spectral analysis
- determination of sodium in water by flame photometry
● Nephelometry
- nefelometric determination of chlorides

3.Electroanalytical methods -
● Voltammetric titration with polarized electrodes
- iodometry with instrumental indication during titration
● Potentiometric titration
- determination of kys. Phosphate titration with sodium hydroxide to II. titration degree.
● Quantitative polarographic analysis
- determination of Zn by the calibration curve method

4.Chromatography techniques -
● Capillary gas chromatography
- identification of substances in an unknown sample using the Kovets index method a practice
work with internal standard
● Reverse phase high performance liquid chromatography
- determination of phenolic compounds in water samples after pre-separation by solid phase
- determination of caffeine in food samples
● Electrophoresis, Thin Film Chromatography
- optimization of separation conditions and identification of synthetic dyestuffs in food

Recommended or required reading:
  • PURDEŠOVÁ, A. – TARAPČÍK, P. – SÁDECKÁ, J. – HROBOŇOVÁ, K. – ŠPÁNIK, I. – RIEVAJ, M. – ŠVORC, Ľ. – MANOVÁ, A. – ČACHO, F. Praktikum z analytickej chémie. Bratislava : Nakladateľstvo STU, 2011. 242 s. ISBN 978-80-227-3555-1.
  • BUSTIN, D. – HLADKY, Z. – GARAJ, J. Analytická chémia. Alfa, Bratislava: SNTL, Praha, 1987. 744 s.
Planned learning activities and teaching methods:
Subject is realized through laboratory exercises.
Assesment methods and criteria:
There will be three written clearances for 30 points during the semester. For an evaluation "A" must be obtained at least 90 points of a total 100 points, "B" at least 80 points, "C" at least 70 points, "D" at least 60 points, "E" at least 50 points. Credits shall not be granted to a student who obtains a written clearance less than 15 points.
Language of instruction:
Slovak, English
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